Data Availability StatementThe datasets used and/or analyzed through the current research are available through the corresponding writer on reasonable demand. . The info are presented as median values with higher and lower quartiles. Anisomycin Categorical variables had been likened using the chi-square check, as well as the MannCWhitney check was employed to compare differences between groups. The Friedman test was used for repeated steps analysis of repeated within-group comparisons for continuous variables, and the Wilcoxon signed-rank test was used for post hoc analysis. All statistical analyses were performed using SPSS software, and a p-value of 0.05 was considered to indicate statistical significance [2, 29, 30]. Acknowledgements The study was sponsored by CMRPG8E0151-CMRPG8E0152. We are thankful for the help by the Center for Shockwave Medicine and Tissue Engineering, Department of Medical Research, Kaohsiung Chang Gung Memorial Hospital, Taiwan. Abbreviations AAOSAmerican Academy of Orthopedic SurgeonsBMIbody mass indexCADcoronary artery diseasePEpulmonary embolismVTEvenous thromboembolismTKAtotal knee arthroplasty Authors contributions JYK and Anisomycin CJW conceptualize the study. KKS and KTW have made substantial contributions to the acquisition and analysis of the study. FSW and WYC interpreted the data. KKS drafted the work, and SJI substantively revised the manuscript. All authors read and approved the final manuscript. Funding The funding sources are from Chang Gung Medical Foundation (CMRPG8G0051, CRRPG8F0461, CRRPG8F0462, CRRPG8F0463 and CLRPG8E0131). Availability of data and materials The datasets used and/or analyzed during the current study are available from the corresponding author on reasonable request. Ethics approval and consent to participate The Rabbit Polyclonal to AQP12 study was approved by the Institutional Review Board of Chang Gung Memorial Hospital (IRB number: 100-0038A3) and registered in ClinicalTrials.gov website (registration number: “type”:”clinical-trial”,”attrs”:”text”:”NCT02206321″,”term_id”:”NCT02206321″NCT02206321). Consent for publication Not applicable in our study. Competing interests The authors declare that they have no Anisomycin competing interests. Footnotes Publisher’s Note Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Contributor Information Ka-Kit Siu, Email: moc.liamg@kksleahcim. Kwan-Ting Wu, Email: moc.liamg@3257ymene. Jih-Yang Ko, Email: wt.gro.hmgc@yjok. Feng-Sheng Wang, Email: ten.tenih.33sm@sfgnaw. Wen-Yi Chou, Email: wt.gro.hmgc@uohcyarrum. Ching-Jen Wang, Email: moc.liamg@516gnaweyaf. Shu-Jui Kuo, Email: moc.liamg@37010409b..